Search scope:
排序： Display mode:
OUYANG Shan, ZHANG Qinghua, QIAO Fuyuan
Frontiers of Medicine 2007, Volume 1, Issue 4, Pages 386-389 doi: 10.1007/s11684-007-0075-6
Keywords： pregnancy concentration possible relationship responsible hepatocyte
Bioartificial liver devices: Perspectives on the state of the art
Yi-Tao DING, MM, Xiao-Lei SHI, MD
Frontiers of Medicine 2011, Volume 5, Issue 1, Pages 15-19 doi: 10.1007/s11684-010-0110-x
Acute liver failure remains a significant cause of morbidity and mortality. Bioartificial liver (BAL) devices have been in development for more than 20 years. Such devices aim to temporarily take over the metabolic and excretory functions of the liver until the patients’ own liver has recovered or a donor liver becomes available for transplant. The important issues include the choice of cell materials and the design of the bioreactor. Ideal BAL cell materials should be of good viability and functionality, easy to access, and exclude immunoreactive and tumorigenic cell materials. Unfortunately, the current cells in use in BAL do not meet these requirements. One of the challenges in BAL development is the improvement of current materials; another key point concerning cell materials is the coculture of different cells. The bioreactor is an important component of BAL, because it determines the viability and function of the hepatocytes within it. From the perspective of bioengineering, a successful and clinically effective bioreactor should mimic the structure of the liver and provide an in vivo-like microenvironment for the growth of hepatocytes, thereby maintaining the cells’ viability and function to the maximum extent. One future trend in the development of the bioreactor is to improve the oxygen supply system. Another direction for future research on bioreactors is the application of biomedical materials. In conclusion, BAL is, in principle, an important therapeutic strategy for patients with acute liver failure, and may also be a bridge to liver transplantation. It requires further research and development, however, before it can enter clinical practice.
Keywords： acute liver failure bioartificial livers hepatocyte bioreactor
Transcription Factors HNF1A, HNF4A, and FOXA2 Regulate Hepatic Cell Protein N-Glycosylation Article
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
Engineering 2024, Volume 32, Issue 1, Pages 58-69 doi: 10.1016/j.eng.2023.09.019
Hepatocyte nuclear factor 1 alpha (HNF1A), hepatocyte nuclear factor 4 alpha (HNF4A), and forkhead
Keywords： Clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) Epigenetics Hepatocytenuclear factor 1 alpha (HNF1A) Hepatocyte nuclear factor 4 alpha (HNF4A) Forkhead box protein A2 (
Zhengyi Jiang, Zeyu Sun, Xiaoxi Ouyang, Yalei Zhao, Menghao Zhou, Baohong Wang, Qirui Li, Linxiao Fan, Sainan Zhang, Lanjuan Li
Engineering 2020, Volume 6, Issue 11, Pages 1302-1314 doi: 10.1016/j.eng.2020.02.011
Primary hepatocytes (PHCs) are widely used in various fields, but the progressive deterioration of liver-specific features in vitro significantly limits their application. While the transcriptional regulation and whole cell proteome (WCP) of PHCs have been extensively studied, only a small number of studies have addressed the role of posttranslational modifications in this process. To elucidate the underlying mechanisms that induce dedifferentiation, we carried out parallel quantifications of the transcriptome, WCP, ubiquitinome, and phosphoproteome of rat PHCs after 0, 6, 12, 24, and 48 h of in vitro culture. Our data constitute a detailed proteomic analysis of dedifferentiated PHCs including 2196 proteins, 2056 ubiquitinated sites, and 4932 phosphorylated peptides. We revealed a low correlation between the transcriptome and WCP during dedifferentiation. A combined analysis of the ubiquitinome with the corresponding WCP indicated that the dedifferentiation of PHCs led to an increase in nondegradative K27 ubiquitination. Functional analysis of the altered phosphoproteins suggested a significant enrichment in ferroptosis. In all, 404 proteins with both ubiquitination and phosphorylation were identified to be involved in critical metabolic events. Furthermore, Ptbp1, Hnrpd, Hnrnpu, and Srrm2 were identified as hub genes. Taken together, our data provide new insights into proteome dynamics during PHC dedifferentiation and potential targets to inhibit the dedifferentiation process.
Keywords： Ubiquitination Phosphoproteome Proteome Dedifferentiation Primary hepatocytes
Title Author Date Type Operation
Relationship between expression of hepatocyte grow factor and apoptosis of trophoblasts in hypertensive
OUYANG Shan, ZHANG Qinghua, QIAO Fuyuan
Journal Article
Bioartificial liver devices: Perspectives on the state of the art
Yi-Tao DING, MM, Xiao-Lei SHI, MD
Journal Article
Transcription Factors HNF1A, HNF4A, and FOXA2 Regulate Hepatic Cell Protein N-Glycosylation
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
Journal Article
京公网安备 11010502051620号
